Composite

Part:BBa_K3102024

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-EG1-ter (secreted EG1)

This T7-RBS-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesizing EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.

This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 362
    Illegal AgeI site found at 452
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None